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KMID : 0363020040340020357
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Journal of Korean Academy of Periodontology
2004 Volume.34 No. 2 p.357 ~ p.366
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Effect of Manganese on primary rat calvarial cell
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김지은
김영준/정현주/김옥수
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Abstract
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Chronic exposure to high levels of manganese leads a pronounce and debilitating disorder known as manganism. Research on the toxic manifestation of manganese have focused primarily on its neurological effects because exposure to high levels of the metal produces a distinct and irreversible extrapyramidal dysfunction resembling the dystonic movements associated with Parkinson's physiological and biochemical systems in the body.
The purpose of this study was to evaluate the effect of manganeses on primary rat calvarial cell growth and toxicity. The experimental groups were in concentration of 0, 10, 30, 60, 100, 300 μM. Cell activity was assessed at day 1 and day 3 using a fluorescent molecular probe. Cell proliferation was evaluated at day 1 and day 3 by MTT assay. The amount of total protein synthesis was measured at day 3 and day 7.
The results were as follows:
The proliferation of primary rat calvarial cells were inhibited by MnCl2 in the concentration exceeding 100 μM. The primary rat calvarial cells treated with MnCl2 showed similar protein synthesis to the control group except in 100 μM.
These result suggest that manganese suppress the viability and protein synthesis of primary rat calvarial cells in concentration exceeding 100 μM.
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KEYWORD
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manganase, calvarial cell, viability
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